- Title
- Regulation of low-density lipoprotein receptor activity by estrogens and phytoestrogens in a HepG2 cell model
- Creator
- Owen, Alice J.; Roach, Paul D.; Abbey, Mavis
- Relation
- Annals of Nutrition and Metabolism: European journal of nutrition, metabolic diseases and dietetics Vol. 48, Issue 4, p. 269-275
- Publisher Link
- http://dx.doi.org/10.1159/000080462
- Publisher
- Karger
- Resource Type
- journal article
- Date
- 2004
- Description
- Background/Aims: Estrogen treatment is thought to lower low-density lipoprotein (LDL) cholesterol levels by increasing clearance through hepatic LDL receptors. This study aimed to determine the effect of estrogens and phytoestrogens on LDL receptor activity in a human hepatoma cell line, HepG2. Methods: HepG2 cells in culture were incubated for 24 h with estrogen or phytoestrogen and LDL receptor activity was measured by examining the cellular binding of colloidal gold-labelled LDL. Results: 17β-Estradiol significantly increased LDL receptor activity whereas estriol had negligible effects. Incubation with the isoflavonoids, formononetin, biochanin A and daidzein, caused significant elevations in receptor activity at concentrations above 40 μM. Coumestrol, a coumestan with a high level of estrogenic activity, caused a 3-fold increase in receptor activity at a concentration of 50 μM. Of the phytoestrogenic mammalian lignans enterolactone and enterodiol, only enterolactone displayed the ability to significantly upregulate LDL receptor activity at 50 μM. Conclusion: This study suggests that the LDL receptor-stimulating effect of natural estrogens is mainly due to estradiol and that the cholesterol-lowering effect of diets high in phytoestrogens may be due in part to their ability to increase hepatic LDL receptor activity.
- Subject
- estrogen; phytoestrogen; low-density lipoprotein; receptor LDL; isoflavone; antioxidant
- Identifier
- uon:897
- Identifier
- http://hdl.handle.net/1959.13/26478
- Identifier
- ISSN:0250-6807
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